Development and validation of HPLC method for the quantification of clotrimazole in a gelatin film formulation

Abstract Background Clotrimazole is used to treat topical vaginal yeast infections. Vaginal gelatin film formulations of clotrimazole were prepared, and the present study aims develop an HPLC method for quantifying in formulation. Results The chromatographic separation components was achieved on a Phenomenex Luna C18(2) (150 × 4.6 mm, 5 μm) stationary phase with Security Guard Cartridge C18 (4 3 mm) using acetonitrile water (70:30% v/v) as mobile phase. flow rate 1.0 ml/min absorbance monitored at 210 nm. eluted 5.6 min. procedures capabilities evaluated against criteria system suitability, specificity, linearity, precision, accuracy, robustness defined by ICH guidelines. showed good linearity ( R 2 = 0.999) range 10–50 μg/ml average recovery 101.05%. Conclusion could be applied quantification